Ultrastructural and morphometric studies on the rat pituitary thyrotrophs and thyroid follicular cells following administration of thyrotropin releasing hormone.

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  • 甲状腺刺激ホルモン放出ホルモン投与後のラット下垂体TSH細胞および甲状腺濾胞細胞の超微形態学的計測的研究
  • 甲状腺刺激ホルモン放出ホルモン投与後のラット下垂体TSH細胞および甲状腺濾胞細胞の超微形態学的計測的研究〔英文〕
  • コウジョウセン シゲキ ホルモン ホウシュツ ホルモン トウヨゴ ノ ラット

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The pituitary thyrotrophs and thyroid follicular cells of rats were studied by electron microscopy and morphometric analysis at 5, 10, 20, 30 and 60min after intravenous injection of 200μg of thyrotropin releasing hormone (TRH) and at 10min after injection of 10μg TRH.<br>Multiple granule extrusions in a group were often observed around the pituitary thyrotrophs at 5, 10 and 20min after administration of 200μg TRH, as well as at 10min after injectiom of 10μg TRH. The number of released granules reached its maximum at 10min after 200μg TRH injection, but the total number of secretory granules in the cytoplasm of thyrotrophs did not show any significant variation throughout the experimental period. Percent area of the rough endoplasmic reticulum indicated the maximum value at 20min and that of the Golgi apparatus at 10min. These findings suggest that TRH stimulates the thyrotroph and accelerates synchronously the secretion as well as the synthesis of TSH.<br>In the thyroid follicular cells after 200μg TRH injection, numerous small vesicles which might be secretory granules were found in the apical cytoplasm at 5 and 10min. Pseudopod formation on the luminal surface and accumulation of colloid droplets and lysosome-like granules in the apical cytoplasm were most frequently observed at 20 and 30min. The value of the mean ratio of the diameter of the colloid lumen to the cell height of follicular cells in the thyroid follicles of all experimental animals stimulated with TRH decreased much more than that of the control animals. This value may indicate the increased activity of follicular cells to reabsorb the colloid.

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