ギンブナのランゲルハンス島の光学および電子顕微鏡的観察

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  • 小林 寛
    Department of Anatomy, Gunma University School of Medicine
  • 高橋 嘉幸
    Department of Anatomy, Gunma University School of Medicine

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タイトル別名
  • Light and Electron Microscope Observations on the Islets of Langerhans in <i>Carassius carassius longsdorfii</i>
  • ギンブナ ノ ランゲルハンストウ ノ コウガク オヨビ デンシ ケンビキョウテキ カンサツ エイブン

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The islet of Langerhans of Carassius carassius longsdorfii (TEMMINCK et SCHLEGEL) has been observed by light and electron microscopy.<br>1. The islet tissue of the Carassius is concentrated into several spherical bodies embedded in the exocrine pancreas or in the mesentery. They are surrounded by a distinct connective tissue capsule, and richly supplied by blood capillaries.<br>2. By light microscopy, A, B and D cells are distinguished according to their staining reactions. The cells deserving the name of “clear” or “agranular” cells under the light microscope can be identified by electron microscopy as D cells containing no or few secretory granules. B cells form a network of cell cords throughout the islets, in the meshes of which A, D and “clear” cells are distributed. The population ratio of A:B:D cells comes to 25:50:25 (of which 10 are the light microscopic “clear cells”).<br>3. Argyrophil islet cells are demonstrated in the silver impregnated specimens and correspond in shape, distribution and number to the D cells.<br>4. Under the electron microscope the three islet cell types are distinguished by the specific ultrastructural features of their secretory granules. The D cells of the Carassius are further characterized by the abundant occurrence of the vesicles and tubules of the agranular endoplasmic reticulum.<br>5. The α-granules of the Carassius (200-400mμ in diameter) consist of a membranous sac encasing a homogeneous spherical core of high electron density with a slight space between the two being characteristic. The β-granules of the Carassius (200-400mμ in diameter) closely resemble those of the carp (TITLBACH, 1966), showing a membranous sac and a spherical core which is composed of a homogeneous substance and needle- or bar-shaped crystalloids; the electron lucent space between the sac and the core is inconspicuous. In the δ-granules of the Carassius (200-400mμ in diameter), a round membrane sac is closely attached to a finely granular or flocculent core of low electron density. A few smaller and denser granules may occur among the ordinary δ-granules and near the Golgi complex; they are regarded as immature α-granules.<br>6. In the Carassius, the formation of secretory granules in the Golgi complex is evidenced in the three islet cell types. The release of secretory granules by means of emiocytosis is confirmed in A and D cells but not in B cells. Findings suggestive of the granule release by intracytoplasmic dissolution are obtained in all three islet cell types but especially conspicuously in B cells.

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