Ferritin as a Surface Marker for Immunoscanning Electron Microscopy

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  • HATTORI Akira
    First Department of Internal Medicine, Niigata University School of Medicine
  • MATSUKURA Yutaka
    Department of Legal Medicine, Niigata University School of Medicine
  • ITO Suiko
    First Department of Internal Medicine, Niigata University School of Medicine
  • FUJITA Tsuneo
    Department of Anatomy, Niigata University School of Medicine
  • TOKUNAGA Junichi
    Department of Microbiology, Kyushu Dental College

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Other Title
  • 免疫走査電子顕微鏡のための表面マーカーとしてのフェリチン
  • メンエキ ソウサ デンシ ケンビキョウ ノ タメ ノ ヒョウメン マーカー ト
  • Observation of Individual Ferritin Particles on Erythrocytes
  • 赤血球表面のフェリチンの観察

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Abstract

This paper comprises a part of our study to examine whether ferritin can be used as an immunological surface marker for high resolution scanning electron microscopy.<br>In the first part of the study individual ferritin particles in a sample of purified ferritin were demonstrated as spheres with a diameter of about 170Å by use of fixation by glutaraldehyde and thin coating by ion sputtering.<br>In the second part of this study, ferritin particles as indicating the antigen sites of blood group A were also recognized on the erythrocytes by the same techniques as in the first part. Ferritin deposition differed in amount among the erythrocytes, but without regard to the shape of the cells. On the individual erythrocytes, heavier depositions tended to occur in the concavity of the cell.<br>In conclusion, ferritin is useful as a surface marker in immunoscanning electron microscopy. It has the benefit, in comparison with the other markers hitherto reported, of allowing us to observe the fine features of the cell surface under immunological reaction.

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