Quantitative Difference of 7S Globulin on Vegetable Soybean Seeds

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  • Abe Toshinori
    Laboratory of Plant Molecular Genetics and breeding, Faculty of Agriculture, Yamagata University
  • Kano Mutsumi
    Laboratory of Plant Molecular Genetics and breeding, Faculty of Agriculture, Yamagata University
  • Sasahara Takeo
    Laboratory of Plant Molecular Genetics and breeding, Faculty of Agriculture, Yamagata University

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  • エダマメ用ダイズ品種における７Ｓグロブリンの量的変異
  • エダマメヨウ ダイズ ヒンシュ ニ オケル 7Sグロブリン ノ リョウテキ ヘンイ

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Abstract

Quantitative difference of the 7S globulin subunits in immature seeds of five vegetable-type (Edamame) and three grain-type cultivars of soybean which were harvested 35 days after flowering were examined by sodium dodecylsulfate-polyacrylamide electrophoresis (SDS-PAGE). In the vegetable type soybean cultivars, both the immature and mature seeds of Shirayama-dadacha and Kanro in which the seeds are shriveled and brown color in feature, showed reduced levels of β-conglycinin β-subunits and sucrose binding proteins. Densitometric scans of SDS-PAGE protein gels from vegetable-type cultivar, Shirayama-dadacha, confirmed that levels of β-conglycinin-β-subunits and sucrose binding proteins were about 70 and 75%, respectively, in comparison with those of grain-type cultivars. These quantitatively reduced levels of 7S globulin leaded to be possible increase of 11S/7S ratio and then be preferable for nutrition value. By two-dimensional gel electrophoresis, the different patterns of β-conglycinin β-subunits were found in vegetable-type cultivars, Shirayama-dadacha and Kanro, compared with those of grain type ones. The protein spots that showed different patterns or expressed reduced levels were examined by amino acid sequence analysis. These protein spots were identified as β-conglycinin β-subunits and sucrose binding proteins.

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