Simultaneous Determination of Ascorbate and Dehydroascorbate by High-Performance Liquid Chromatography Using ODS Column

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  • ODS 系カラムによるビタミン C の高速液体クロマトグラフィー分析
  • ODSケイ カラム ニ ヨル ビタミン C ノ コウソク エキタイ クロマトグラフィー ブンセキ

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Abstract

Simultaneous determination of ascorbate and dehydroascorbate by high-performance liquid chromatography was examined. Based on the method of Yasui et al. (1991), the most suitable column for good separation was selected from various ODS columns and separation modes not described in the original report. Using the Imtakt Unison UK-C18 (3 μm, 4.6 mm × 150 mm) column, with 2 mM HClO4 at 1.0 ml/min as an eluent, 100 mM NaOH and 100 mM NaBH4 at 0.5 ml/min as a post-column reaction, and UV detection at 300 nm, the separation of the peaks was greatly improved when compared with the original method, and analysis time was shortened.

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