Analysis of Disulfide Bonds in Soybean Proteins using Fluorescence Labeling by Monobromobimane (mBBr)

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  • 蛍光色素モノブロモバイメイン標識による大豆タンパク質ジスルフィド結合の解析
  • ケイコウ シキソ モノブロモバイメイン ヒョウシキ ニ ヨル ダイズ タンパクシツ ジスルフィド ケツゴウ ノ カイセキ

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Disulfides bonds in soybean proteins were estimated by labeling with a fluorescent reagent, monobromobimane (mBBr) and subsequent electrophoresis. Prior to the labeling by mBBr, disulfide bonds in the proteins were chemically reduced by 0.5mM dithiothreitol (DTT) at room temperature. Acidic and basic subunits of glycinin, and Bowman-Birk protease inhibitors were detected as major bands in the soybean proteins. It is found that fluorescence intensity of basic subunit of glycinin correlated significantly to the rupture stress of Tofu using 33 varieties of domestic soybean.

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