Detection of Antibodies against Striped Jack Nervous Necrosis Virus (SJNNV) from Brood Stocks of Striped Jack.

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  • Mushiake Keiichi
    Komame Station of Japan Sea-Farming Association
  • Arimoto Misao
    Goto Station of Japan Sea-Farming Association
  • Furusawa Toru
    Western Division of Japan Sea-Farming Association
  • Furusawa Iwao
    Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University
  • Nakai Toshihiro
    Laboratory of Fish Pathology, Faculty of Applied Biological Science, Hiroshima University
  • Muroga Kiyokuni
    Laboratory of Fish Pathology, Faculty of Applied Biological Science, Hiroshima University

Bibliographic Information

Other Title
  • シマアジ親魚からの神経壊死症原因ウイルス(SJNNV)に対する抗体の検出
  • シマアジ新魚からの神経壊死症原因ウイルス(SJNNV)に対する抗体の検出〔英文〕
  • シマアジ シンギョ カラ ノ シンケイ エシショウ ゲンイン ウイルス SJ

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Abstract

It has been indicated that parental spawners were a source of infection of a viral disease, “viral nervous necrosis”, in larval striped jack Pseudocaranx dentex. In this study, the prevalence of antibodies against the causative virus (SJNNV) was examined among brood stocks of striped jack by indirect ELISA. Indirect ELISA using purified SJNNV, rabbit anti-SJNNV serum, and enzyme-conjugated goat anti-rabbit IgG antibody was employed for antibody detection from the plasma. The plasma IgM partially purified by ion-exchange chromatography was used for ELISA detection. The antibody to SJNNV was detected at high frequency (65%) in plasma samples collected from brood stocks reared at various facilities regardless of their sex or origin (wild or domestic). This indicates that the virus is prevalent among cultured populations of this fish species.

Journal

  • NIPPON SUISAN GAKKAISHI

    NIPPON SUISAN GAKKAISHI 58 (12), 2351-2356, 1992

    The Japanese Society of Fisheries Science

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