Development of the Maturation- and Ovulation-induction Method in Cultured Tiger Puffer Takifugu rubripes by Hormonal Treatments.
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- Chuda Hisashi
- Nagasaki Prefectural Institute of Fisheries
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- Matsuyama Michiya
- Faculty of Agriculture, Kyushu University
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- Ikeda Yoshihiro
- Nagasaki Prefectural Institute of Fisheries
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- Matsuura Shuhei
- Faculty of Agriculture, Kyushu University
Bibliographic Information
- Other Title
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- トラフグ養成親魚からの採卵技法の開発
- トラフグ ヨウセイ オヤウオ カラ ノ サイラン ギホウ ノ カイハツ
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Abstract
We developed an effective method for the induction of oocyte maturation and ovulation in the cultured tiger puffer Takifugu rubripes by hormonal treatments, in order to supply the fertilized eggs for seedling production. Ovarian biopsy just prior to the hormonal treatments revealed the oocyte diameter and developmental stages of the oocytes.<br>A luteinizing hormone-releasing hormone analogue (LHRH-a) cholesterol pellet (400μg/kg) implantation successfully induced oocyte maturation for three-year-old cultured tiger puffer which had yolk accumulated ovaries. Thereafter, single injection of HCG (500IU/kg) combined with chum salmon pituitary (SP) homogenate (7mg/kg) was applied to the fish to synchronize ovulation among fish. Ovulation mainly occurred between 96 and 120 hours after HCG+SP injection. Thus, a technique for obtaining eggs of high quality from the cultured tiger puffer was developed.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 63 (5), 728-733, 1997
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001206410997504
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- NII Article ID
- 10004851860
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
- http://id.crossref.org/issn/00215392
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- NDL BIB ID
- 4305150
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed