Biochemiacal Identification of Two Gempylid Fishes Causative of a Food Poisoning

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  • OCHIAI Yoshihiro
    Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo
  • WATABE Shugo
    Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo
  • HASHIMOTO Kanehisa
    Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo
  • NARITA Hiroko
    Shizuoka Prefectural Institutel of Public Health Health and Environmental Science
  • UKISHIMA Yoshiyuki
    Shizuoka Prefectural Institutel of Public Health Health and Environmental Science
  • NARA Masato
    Shizuoka Prefectural Institutel of Public Health Health and Environmental Science

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Other Title
  • アブラソコムツ，バラムツなど食中毒原因魚種の生化学的鑑別
  • アブラソコムツ,バラムツ ナド ショクチュウドク ゲンイン ギョシュ ノ セイ

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Attempts were made to identify biochemically two gampylid fishes, escolar (“aburasokomutsu”) Lepidocybuum Flavobrunneum and castor oil fish (“baramutsu”) Ruvettus pretiosus, whose muscles contain a large amount of wax esters and hence may casuse a food poisoning when ingested. Myogen fractions were prepared from the ordinary muscles of both fishes as well as various fishes, and subjected to polyacrylamide gel electrophoresis, followed by staining with Coomassie brilliant bule for protein and with reagents for lactate and malate dehydrogenase activities. Results showed that the protein pattern is species-specific enougy to distinguish the two gempylid fishes from any of the other fishes tested. The pattern of either dehydrogenase was found to be less species-specific. However, simultaneous analyses of MDH and protein patterns faciliated the identification of buth gempylid fishes. Cellulose acetate membrane electrophophoresis was also found to be applicable for this purpose, though less satisfactorily.

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