Cultivation conditions for subtilisin inhibitor-producing bacterium and general properties of the inhibitor "Marinostatin".
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- IMADA Chiaki
- Ocean Research Institute, University of Tokyo
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- TAGA Nobuo
- Ocean Research Institute, University of Tokyo
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- MAEDA Masachika
- Ocean Research Institute, University of Tokyo
Bibliographic Information
- Other Title
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- サブチリシンインヒビター生産菌培養条件の検討およびインヒビター“Marinostain”の諸性状
- サブチリシンインヒビター生産菌培養条件の検討およびインヒビター"Marinostain"の諸性状〔英文〕
- サブチリシン インヒビター セイサンキン バイヨウ ジョウケン ノ ケントウ
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Abstract
The marine Alteromonas sp. B-10-31 has been found to produce a very strong extracellular subtilisin inhibitor. We named this inhibitor “Marinostatin” and the optimal medium of strain B-10-31 for the production of Marinostatin was studied. Among various different nitrogen and carbon sources, 0.6% (w/v) polypepton (Daigo) proved to be the most effective nitrogen source and 0.05% (w/v) glucose stimulated the bacterial growth and production of Marinostatin. The maximum production (22.0 Unit/ml) was obtained at pH 6.0 in full strength seawater at 20°C and shaking for 24-33 hrs. The production of Marinostatin was almost parallel to the growth of the strain used. Unlike other protease inhibitors reported so far, Marinostatin was unstable in the stationary phase of growth due to the production of a proteolytic enzyme by the strain.<br>The crude Marinostatin was thermostable and showed a wide range of pH stability. It also exhibited inhibitory activity against a wide variety of proteases.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 51 (5), 805-810, 1985
The Japanese Society of Fisheries Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001206412630272
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- NII Article ID
- 130001543274
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- NII Book ID
- AN00193422
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- COI
- 1:CAS:528:DyaL2MXktlClsrk%3D
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- ISSN
- 1349998X
- 00215392
- http://id.crossref.org/issn/00215392
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- NDL BIB ID
- 3035189
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed