OMV感染RTG-2細胞およびサケ O. keta 肝細胞の超微構造
書誌事項
- タイトル別名
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- Ultrastructures of OMV infected RTG-2 cells and hepatocytes of chum salmon Oncorhynchus keta.
- OMV感染RTG‐2細胞およびサケO.keta肝細胞の超微構造
- OMV カンセン RTG-2 サイボウ オヨビ サケ O . keta カン
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The ultrastructures of RTG-2 cells and hepatocytes of chum salmon (Oncorhynchus keta) infected with Oncorhynchus mason virus (OMV) were observed by thin sectioning and negative staining. RTG-2 cells were fixed 3 and 7 days after OMV infection (m.o.i.=0.01) and the tissue samples from the liver of moribund 80-day-old, 3-month-old and 4-month-old chum salmon infected with OMV by the immersion method (100 TCID50/ml, 60 min) were prepared for electron microscopy. The infected RTG-2 cells and hepatocytes were prefixed with paraformaldehyde-glutalaldehyde and post fixed with 2% osmic acid.<br>Electron micrographs of both RTG-2 cells and hepatocytes infected with OMV revealed the following activities: nuclei showed the margination and the subsequent aggregation of chromatine; syncytia was found in both RTG-2 cells and hepatocytes; the nuclear membranes became smooth and the virus matured in the nucleus of those cells; the matured virus particles were observed in the vesicle and around the cell membrane; and enveloped virus particles were seen in the focus of the hepatocyte. The size of enveloped virus particles was approximately 200×240nm and the nucleocapsid was 115nm in diameter.
収録刊行物
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- 日本水産学会誌
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日本水産学会誌 53 (1), 47-55, 1987
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001206413161728
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- NII論文ID
- 130001542870
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- NII書誌ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- HANDLE
- 2115/38616
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- NDL書誌ID
- 3123014
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可