マダコ肝臓からのβ-<i>N</i>-アセチルヘキソサミニダーゼの分離とその酵素的性質

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タイトル別名
  • Purification and enzymatic properties of .BETA.-N-acetylhexosaminidase from common octopus liver.
  • マダコ肝臓からのβ-N-アセチルヘキソサミニダーゼの分離とその酵素的性質
  • マダコ カンゾウ カラ ノ ベータ N アセチルヘキソサミニダーゼ ノ ブンリ

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抄録

The liver of common octopus Octopus vulgaris contained extremely high activity of β-N-acetyl-hexosaminidase which is useful for structural and functional studies of complex carbohydrates. Asimple procedure has been devised for the isolation of β-N-acetylhexosaminidase from the liver inan electrophoretically homogeneous form. The procedure involved heat treatment at 68°C for 20min, Sephadex G-200 gel filtration, and CM-Sephadex C-50 chromatography. The purified enzyme was sufficiently free from other glycosidases and proteases. The molecular weight of the enzyme was estimated to be 147, 000 by Sephadex G-200 gel filtration. The enzyme exhibited the optimum pH at pH 5.0 and was stable between pH 4 and 8. For p-nitrophenyl β-N-acetylgluco-saminide, Km was 0.63mM and Vmax was 192μmol/min•mg.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 53 (6), 1025-1031, 1987

    公益社団法人 日本水産学会

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