動揺の激しい船内でのＡＴＰ関連化合物の分離定量法　　逆相分配カラムによる高速液体カラムクロマトグラフィー法

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タイトル別名
  • Method of quantitative analysis of ATP related compounds on the rough sea - Method of high-performance liquid chromatography using reversed-phase column.
  • ドウヨウ ノ ハゲシイ センナイ デ ノ ATP カンレン カゴウブツ ノ ブ

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抄録

The separation and the quantitative analysis of ATP, ADP, AMP, IMP, inosine and hypoxanthine in the fish muscle were made using the high-performance liquid chromatography with the reversed-phase column.<br>All the ATP related compounds as well as most of the other nucleic acid related compound could be eluted under the phosphate buffer (0.05M-KH2PO4: 0.05M-K2HPO4=1:1) at pH6.78 with the reasonable resolution and the retention time. For the ATP related compounds in the extract of fish muscle, the recovery and the reproducibility by this method were satisfactory and the values obtained agreed with those prepared by the general purpose method of ion exchange chromatography.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 51 (8), 1363-1369, 1985

    公益社団法人 日本水産学会

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