魚類筋肉ミオキナーゼに関する研究 I. : コイ筋肉よりミオキナーゼの分離・精製

大城 善太郎, 福島 正和, 林 征一

doi:10.2331/suisan.40.291

魚類筋肉ミオキナーゼに関する研究 I. : コイ筋肉よりミオキナーゼの分離・精製

DOI 日本農学文献記事索引 Web Site

大城善太郎

鹿児島大学水産学部
福島正和

鹿児島大学水産学部
林征一

鹿児島大学水産学部

書誌事項

タイトル別名

魚類筋肉ミオキナーゼ　Ｉ　　コイ筋肉よりミオキナーゼの分離・精製
Studies on Myokinase in the Muscle of Fishes-I
コイキンニクヨリミオキナーゼノブンリセイセイ
コイ筋肉よりミオキナーゼの分離・精製

この論文をさがす

説明

Myokinase (ATP: AMP phosphotransferase, EC 2.7.4.3) catalysing the equilibrium among ATP, ADP and AMP, was isolated from the extract of carp muscle by ammonium sulfate fractionation, Sephadex G-75, and by DEAE-cellulose and CM-cellulose column chromatography.<br>The separation of two distinct forms of myokinase into different fractions was brought about by gel filtration on Sephadex G-75. These enzymes were termed myokinase I and myokinase II in order of elution from the column.<br>Myokinase I was further purified by means of DEAE-cellulose column chromatography, while myokinase II was further purified on CM-cellulose columns. These purified myokinase isozymes appeared homogeneous on disc gel electrophoretic analysis.<br>This fact suggested that, in spite of the seeming identity of the substrate specificities of these two enzymes, these two forms of myokinase isozymes are composed of different types of proteins.