Heat-induced DNA injury and its repair in Micrococcus radiodurans.

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  • UCHIDA Aritsune
    Laboratory of Microbiology, Department of Fisheries, Faculty of Agriculture, Kyoto University
  • HARADA Kazuki
    Laboratory of Microbiology, Department of Fisheries, Faculty of Agriculture, Kyoto University
  • KADOTA Hajime
    Laboratory of Microbiology, Department of Fisheries, Faculty of Agriculture, Kyoto University

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Other Title
  • <i>Micrococcus radiodurans</i>における加熱によるDNAの損傷とその修復
  • Micrococcus radioduransにおける加熱によるDNAの損傷とその修復〔英文〕
  • Micrococcus radiodurans ニ オケル カネツ ニヨル D

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Abstract

When Micrococcus radiodurans cells were heated at 55°C for 8min, DNA from the cells sedimented in an alkaline sucrose density gradient more slowly than DNA from unheated cells as DNA from the bacteria exposed to ionizing radiation. The single strand scissions in DNA molecules were repaired during incubation in nutrient broth. Nalidixic acid inhibited such a repair during post-heating incubation.

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