Application of protease isolated from an actinomycete for Nemopilema nomurai Kishinouye decomposition

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  • DOI HIROYASU
    Microbial Chemistry Research Foundation, Specified Research Promoting Group
  • OKA TATSUZO
    Department of Veterinary Pathobiology, Faculty of Agriculture, Kagoshima University
  • NONOMURA YOSHIAKI
    Microbial Chemistry Research Foundation, Specified Research Promoting Group

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  • 放線菌が産生するプロテアーゼによるエチゼンクラゲの分解への適用
  • ホウセンキン ガ サンセイスル プロテアーゼ ニ ヨル エチゼンクラゲ ノ ブンカイ エノ テキヨウ

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Abstract

  The bacterial strain 99-GP-2D-5 producing protease (E77) was isolated from Yatsuhigata tidal flat (Yatsuhigata) in Chiba Prefecture and its taxonomic classification was investigated in detail. From the result of molecular phylogenetic analysis based on base sequences of 16S rDNA, and amino acid analysis of the cell wall peptidoglycan, the bacterium was identified as an actinomycete of the genus Streptomyces. The optimal temperature and pH for enzyme activity were 70°C and 8, respectively. E77 could completely decompose Nemopilema nomurai Kishinouye under stable conditions (50°C and pH 8) within 60 min.<br>

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