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- TSUCHIYA Takahide
- Department of Chemistry, Sophia University
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- YAMADA Noboru
- Department of Chemistry, Sophia University Central Research Laboratory, Midorijuji Co., Ltd.
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- MORI Hisayo
- Department of Chemistry, Sophia University
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- MATSUMOTO Juichiro J.
- Department of Chemistry, Sophia University
Bibliographic Information
- Other Title
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- イカ筋ミオシンのATPase活性
- イカ筋ミオシンのATPase活性〔英文〕
- イカ キン ミオシン ノ ATPase カッセイ エイブン
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Description
Myosin of the obliquely striated mantle muscle of squid, Ommastrephes sloani pacificus, was isolated by the authors' method and its ATPase profiles were studied under various reaction con-ditions. With Ca2+ (5mM) or Mg2+ (1mM) present and in 600mM KCI, the activity-pH curve showed 2 peaks, at pH 7.0 and near pH 8.5, where the activity with Ca2+ was higher than that with Mg2+. The activity-ionic strength curve gave a peak at 0.2M KCI with declining slope beyond that concentration. Ca2+ activated the ATPase with a maximum effect at 1-9mM, while Mg2+ caused a slight activation with a maximum effect at 0.1mM. The activity was depressed with EDTA for all concentrations between 0.1-1mM, but in a different manner from the ATPase of skeletal muscle myosins. PCMB did not affected either Ca2+-ATPase or EDTA-ATPase. These profiles of the squid myosin ATPase were compared with those of the myosin ATPases from other sources. The squid myosin ATPase was found to be very different from the skeletal muscle myosin ATPases and, in some detailed points, distinct from the myosin ATPases from invertebrate and nonmuscular sources.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 44 (3), 203-207, 1978
The Japanese Society of Fisheries Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001206415797632
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- NII Article ID
- 130001547950
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- NDL BIB ID
- 1935372
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed