Influence of KCl concentrations on the denaturation of carp F-actin.

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  • コイＦ‐アクチンの変性におよぼすＫＣｌ濃度の影響
  • コイF-アクチンの変性におよぼす KCl 濃度の影響
  • コイ F-アクチン ノ ヘンセイ ニ オヨボス KCl ノウド ノ エイキョウ

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Abstract

To study the influence of KCl concentrations on denaturation of carp F-actin, F-actin solutions were stored at 3°C in each KCl solution of 0.1, 0.6 and 1.0M. Viscosity, total and reactive SH groups, and bound nucleotide were measured during storage, and the protective effects of K2-Na1 phosphate buffer, sodium glutamate and ATP on salt denaturation were investigated.<br> The reduced viscosity and the bound nucleotide drastically decreased in the 0.6 and 1.0M KCl solutions, and in the case of the 0.6M KCl solution, the decrease in the reduced viscosity occurred within 3-5h. The total SH groups gradually decreased during storage and the reactive SH groups increased by 2 mol SH/mol of G-actin within 20h after introduction into the 0.6M KCl solution. Three additives, such as ATP, phosphate buffer and sodium glutamate showed protective effects on denaturation and the appearance of latent SH groups was prevented in these cases. In the case of the synthetic actomyosin, reconstituted by mixing myosin and F-actin which were stored separately in 0.6M KCl solutions, actin-activated Mg-ATPase activity decreased during storage. However, myosin Ca-ATPase activity (in the presence of 0.5M KCl) remained at its original value.<br> The above findings show that the structure of carp F-actin is easily changed from its fila-mentous state to small-sized actin or aggregations under higher KCl concentrations, the release of bound nucleotide from actin occurs, and that the latent SH groups of actin molecules appear as a result of denaturation.

Journal

  • NIPPON SUISAN GAKKAISHI

    NIPPON SUISAN GAKKAISHI 54 (11), 2037-2042, 1988

    The Japanese Society of Fisheries Science

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