電位・電流固定法によるミリポアＤＯＰＨ膜の電流‐電圧特性

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  • Current-voltage characteristics of Millipore DOPH membranes by voltage-current clamp method.
  • デンイ デンリュウ コテイホウ ニ ヨル ミリポア DOPH マク ノ デンリ

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The Millipore DOPH model membrane is composed of a Millipore filter whose pores are filled with a lipid analogue, dioleylphosphate (DOPH). The electric resistance of the Millipore DOPH membrane is increased in the mixed salt solution such as KCl and CaCl2, and it oscillates rhythmically under which a current and a pressure stimulus are applied simultaneously. This paper showed electric current (I) -voltage (V) characteristics to discuss the plausible mechanism of the resistance oscillation, because I-V characteristics were one of important properties to described the mechanism of an electric oscillation, and these had not been examined in the Millipore DOPH membrane. The current and voltage responses were observed by using the current or voltage clamp circuits which were made by us. The current or the voltage increased linearly till 0.15 μA or 400mV, but above its oscillatory changes appeared. These meaned that it was difficult to discuss the oscillation mechanism with only I-V characteristics as compared with living cell membranes. The Millipore DOPH membrane consists of many small membranes such as oil droplets, spherical micelles and multilayers formed in filter pores. The oscillations observed under clamps come from this complex structure. From I-V characteristics, however, the structure of the Millipore DOPH membrane could be demonstrated by the simplest model connected with capillaries. Using the capillary model it could be easy illustrated that the sustained oscillation was caused by the conformational change of the small membrane in one capillary.

収録刊行物

  • 膜

    膜 12 (1), 42-48, 1987

    日本膜学会

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