Cultured Cell Sheets Supported by Microporous Membranes of Biodegradable Polyesters

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  • Minbu Hiromi
    Department of Materials Science and Technology, Niigata University
  • Tanaka Takaaki
    Department of Materials Science and Technology, Niigata University

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Other Title
  • 生分解性ポリエステル多孔質膜に支持された培養細胞シート
  • セイブンカイセイ ポリエステル タコウシツマク ニ シジ サレタ バイヨウ サイボウ シート

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Description

Culture of autologous cell sheets is one of the promising methods in regenerative medicine. Cultured epidermis is used for the coverage of massive burn wounds and cultured periosteum is for the treatment of infrabony periodontal defects. In cell sheet engineering cardiomyocyte sheets prepared with temperature–responsive culture dishes are layered to construct cardiac grafts. Those cultured cell sheets, however, need high techniques in culture and/or surgery. We have been developing several microporous membranes of biodegradable polyesters to handle cultured cell sheets easily. Microporous surfaces of poly(L–lactic acid) (PLLA) membrane, which was prepared by thermally induced phase separation method, anchored periosteal tissue segments on the membranes. The cultured periosteal cell sheets on the membranes were successfully osteoinduced in an induction medium. The PLLA membrane implanted with the cell sheet was gradually degraded in a nude mouse without severe inflammation or fibrous encapsulation. Ductility of the membrane with a microporous surface was conferred by blending brittle PLLA with ductile poly(ε–caprolactone) (PCL). Established osteoblast–like (Saos–2) cells grew on a flexible microporous membrane of PCL and hydroxyapatite, the latter of which is a major mineral component of bone and used in bone defect treatment, although the cells grew in a single layer. A finger–like structure of PLLA microporous membrane prepared by nonsolvent–induced phase separation method with a surfactant enhances the cell growth in the membrane possibly by higher diffusion of oxygen, nutrients, and wastes. The biodegradable microporous sheets including those described in the review will complement the existing cultured cell sheet technology.

Journal

  • MEMBRANE

    MEMBRANE 40 (3), 124-129, 2015

    THE MEMBRANE SOCIETY OF JAPAN

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