Cationic Liposome Inhibits Gene Expression in an E.coliCell–Free Translation System
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- Thi Bui Huong
- Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
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- Umakoshi Hiroshi
- Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
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- Suga Keishi
- Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
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- Tanabe Tomoyuki
- Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
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- Shimanouchi Toshinori
- Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
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- Kuboi Ryoichi
- Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
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The role of liposome addition on the gene expression of green fluorescent protein (GFP)was studied in an in vitroprotein translation system in the presence of cationic liposome, prepared by 1,2–dioleoyl–3–trimethyl–ammonium–propane (DOTAP). GFP fluorescence after gene expression was reduced with an increase in DOTAP concentrations,and no fluorescence was observed at certain concentrations of DOTAP. It was found that neither translated GFP nortranscript mRNA for GFP were produced at the above conditions. The above results may show that the DOTAP lipo-some canaffect the inhibition at the transcription step of the GFP synthesis process in addition to its translation andfolding steps.
収録刊行物
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- 膜
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膜 34 (3), 146-151, 2009
日本膜学会
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詳細情報 詳細情報について
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- CRID
- 1390001206421695360
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- NII論文ID
- 10026336035
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- NII書誌ID
- AN0023215X
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- ISSN
- 18846440
- 03851036
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- NDL書誌ID
- 10316579
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
