CRFK細胞でのイヌコロナウイルスの増殖
書誌事項
- タイトル別名
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- Multiplication of canine coronavirus in CRFK cells.
- CRFK細胞でのイヌコロナウイルスの増殖〔英文〕
- CRFK サイボウ デ ノ イヌ コロナウイルス ノ ゾウショク エイブン
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抄録
Canine coronavirus produced CPE in CRFK cells without any blind passages. Syncytium formation was one of the most prominent cytopathic changes after inoculation at a low multiplicity of infection (MOI) of 1 TCID50/cell. At a high MOI of 50 TCID50/cell, however, rounding and detachment of the cells occurred, but no syncytium formation was found. Specific immunofluorescence and immunoperoxidase staining were confined in the perinuclear cytoplasm at 4 hr postinfection when infective titers of culture fluid began to increase. Virus titers reached their maxima of 104.2 and 105.3 TCID50/0.1ml after infection at an MOI of 1 and 50 TCID50/cell respectively. Electron microscopic observation revealed that virus particles occurred in the restricted area of the perinuclear cytoplasm, mainly at the Golgi field, in the early stage of infection. Localization of virus particles as well as positive immunofluorescence and immunoperoxidase staining for specific CCV antigen was seen in the same area of infected cells, i.e., around the centriole and Golgi complex and perinuclear cytoplasm in an indentation in the early stage of infection.
収録刊行物
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- 日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
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日本獸醫學雜誌(The Japanese Journal of Veterinary Science) 48 (6), 1063-1070, 1986
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206421729536
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- NII論文ID
- 110003917461
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- NII書誌ID
- AN00191788
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- COI
- 1:STN:280:DyaL2s7kt1Cjsg%3D%3D
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- ISSN
- 18811442
- 00215295
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- NDL書誌ID
- 3122838
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- PubMed
- 3820910
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可