書誌事項
- タイトル別名
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- Establishment of an attenuated strain of bovine respiratory syncytial virus for live virus vaccine.
- 生ワクチン用牛RSウイルス弱毒株の樹立〔英文〕
- ナマワクチンヨウ ウシ RS ウイルス ジャクドクカブ ノ ジュリツ エイブン
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説明
To develop a live virus vaccine for the prevention of bovine respiratory syncytial (BRS) virus infection in calves, an attempt was made to produce an attenuated virus. The RS-52 strain of BRS virus, isolated from the nasal secretions of a naturally infected calf, was subjected to serial passages in adult hamster lung established (HAL) cells at 30°C and the attenuated rs-52 strain as a live virus vaccine was established. The rs-52 strain multiplied better at 30°C than at 34 or 37°C in HAL cells. The differences in the highest virus titers of this strain between the culture temperature of 30°C and that of 34 or 37°C were more than 2.25 log TCID50. Colostrum-deprived newborn calves and 2-4 months old calves inoculated with the rs-52 strain manifested no abnormal clinical sings at all. However, all inoculated calves produced serum neutralization antibody. When the colostrum-deprived newborn calves immunized with the rs-52 strain were challenged with the virulent NMK7 strain of BRS virus, they exhibited no pyrexia or other abnormal clinical signs at all. An attempt was made to recover the virus from nasal secretions of these calves, but in vain. On the other hand, a nonimmunized control colostrum-deprived newborn calf developed slight fever, mild cough, and slight serous nasal discharge after challenge exposure. The virus was recovered from nasal secretions of this calf. From these results, it was considered that the rs-52 strain could be used as an attenuated live virus vaccine for prevention of BRS virus infection.
収録刊行物
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- 日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
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日本獸醫學雜誌(The Japanese Journal of Veterinary Science) 52 (4), 695-703, 1990
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206421944192
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- NII論文ID
- 110003918254
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- NII書誌ID
- AA10521639
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- COI
- 1:STN:280:DyaK3czmt1Gksg%3D%3D
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- ISSN
- 18811442
- 00215295
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- NDL書誌ID
- 3683464
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- PubMed
- 2391771
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- 使用不可