Binding of Phosphotyrosine to Gallium-Ion-Immobilized Porous Hollow-Fiber Membrane

DOI Web Site 24 References
  • Monma Yuki
    Department of Applied Chemistry and Biotechnology, Chiba University
  • Umeno Daisuke
    Department of Applied Chemistry and Biotechnology, Chiba University
  • Saito Kyoichi
    Department of Applied Chemistry and Biotechnology, Chiba University
  • Sugo Takanobu
    Environment Purification Research Institute Co.

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Other Title
  • ガリウムイオン固定多孔性中空糸膜へのリン酸化チロシンの吸着
  • ガリウムイオン コテイ タコウセイ チュウクウシ マク エ ノ リン サンカ チロシン ノ キュウチャク

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Abstract

Gallium (Ga) ions as an affinity ligand were immobilized to the polymer chain grafted onto a porous hollow-fiber membrane to specifically capture phospho-compounds. An epoxy-group-containing vinyl monomer, glycidyl methacrylate, was graft-polymerized to an electron-beam-irradiated porous hollow-fiber membrane. The partial conversion of the epoxy group into an iminodiacetate group was effective in increasing the molar conversion in the subsequent introduction of a nitrilotriacetate group capable of forming a complex with Ga ions. The amount of Ga ions immobilized was 0.55 mmol per gram of the membrane. The overall rate of adsorption of phosphotyrosine to the Ga-ion-immobilized porous hollow-fiber membrane increased with an increase in the rate of permeation of phosphotyrosine solution through the pores because of the negligible diffusional mass-transfer resistance of phosphotyrosine to the affinity ligand.

Journal

  • MEMBRANE

    MEMBRANE 35 (5), 242-247, 2010

    THE MEMBRANE SOCIETY OF JAPAN

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