リポタンパク質粒子ChylomicronとDisk–HDLモデルの 形成とその機能

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  • Reconstitution of Distinct Lipoprotein Models, Chylomicron and Disk–High–Density Lipoprotein (Disk–HDL)
  • 特集 第37年会生体膜特別講演 リポタンパク質粒子ChylomicronとDisk-HDLモデルの形成とその機能
  • トクシュウ ダイ37ネンカイ セイタイマク トクベツ コウエン リポタンパクシツ リュウシ Chylomicron ト Disk-HDL モデル ノ ケイセイ ト ソノ キノウ

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A: Thermodynamic stability of disk–HDL was evaluated to be more stable than the starting mixture of free apoA–I and phosphatidylcholine (PC) large unilamellar vesicle (LUV). However, the rate of disk formation was very slow, suggesting the large kinetic barrier. Addition of sphingomyelin led to rapid microsolubilization of LUV and formation of the disk. Conformational change of apoA–I in acidic condition also resulted in progressive transformation of PC LUV to the disk. These findings raise the possibility that ABCA1 may act to lower the kinetic barrier and facilitate the disk–HDL generation. B: Cholesterol (Chol)–rich lipid emulsion (triolein–PC/Chol emulsion) was prepared as a model for chylomicron remnant. Internalization of the emulsion particles into macrophages induced leakage of lysosomal protease, cathepsin–L, to cytosol and cell death. This observation implied that Chol of chylomicron remnant plays a role in accelerating atherosclerosis.

収録刊行物

  • 膜

    膜 40 (5), 230-234, 2015

    日本膜学会

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