Membrane properties of dipalmitoyl bis(monoacylglycero)phosphate

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  • Hayakawa Tomohiro
    Lipid Biology Laboratory, RIKEN
  • Makino Asami
    Supra-Biomolecular System Research Group, RIKEN Frontier Research System INSERM-RIKEN Lipidomics Unit
  • Michaud Sabine
    INSERM-RIKEN Lipidomics Unit ICBMS, VMR VCB-CNRS-INSA-CPE 5246, INSA-Lyon, Laboratoire de Chimie Organique
  • Lagarde Michel
    INSERM-RIKEN Lipidomics Unit Inserm UMR 870, INRA U1235, INSA-Lyon, Universite Lyon 1 and Hospices Civils de Lyon
  • Douteau Alain
    ICBMS, VMR VCB-CNRS-INSA-CPE 5246, INSA-Lyon, Laboratoire de Chimie Organique
  • Ito Kazuki
    Structural Materials Science Laboratory, RIKEN SPring-8 Center
  • Hirai Mitsuhiro
    Department of Physics, Gunma University
  • Kobayashi Toshihide
    Lipid Biology Laboratory, RIKEN Supra-Biomolecular System Research Group, RIKEN Frontier Research System INSERM-RIKEN Lipidomics Unit Inserm UMR 870, INRA U1235, INSA-Lyon, Universite Lyon 1 and Hospices Civils de Lyon

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タイトル別名
  • Membrane Properties of Dipalmitoyl Bis (monoaclyglycero) phosphate

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Bis(monoaclyglycero)phosphate (BMP), also known as lysobisphosphatidic acid (LBPA), is a unique phospholipid highly enriched in a specific membrane domain of late endosomes. We synthesized 3,3’-dipalmitoyl bis (monoacylglycero) phosphate (diC16:0 BMP) with naturally occurring sn–1, sn–1’ stereoconfiguration and characterized the thermotropic phase behavior and the membrane properties by using differential scanning calorimetry (DSC), smalland wide-angle X-ray scattering, steady-state fluorescence anisotropy measurement and epifluorescence microscopy of monolayers. In DSC, DiC16:0 BMP displayed a higher melting temperature (52.0 ℃) and a higher molar enthalpy (17.3 kcal/mol) of main phase transition than those of corresponding PG (40.1 ℃ and 9.4 kcal/mol respectively). DiC16: 0 BMP also exhibited weakly energetic broad peak at 19.5 ℃. Wide-angle X-ray scattering revealed that the diC16: 0 BMP undergoes quasi-crystalline phase → tightly packed and partly oriented gel phase → liquid crystalline phase between 10 ℃ and 60 ℃ by passing two calorimetric transitions. Steady-state anisotropy measurements of 1,6- diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) in diC16:0 BMP also indicated a high main transition temperature in BMP. The domain formation in monolayer showed that diC16: 0 BMP formed anisotropic domain shape while PG formed relatively circular domains. The present results, together with our recent results (Hayakawa, T., Hirano, Y., Makino, A., Michaud, S., Lagarde, M., Pageaux, JF., Doutheau, A., Ito, K., Fujisawa, T., Takahashi, H. and Kobayashi, T. (2006) Biochemistry, , 9198-9209) suggest the formation of strong inter-molecular hydrogen bonding between the BMP molecules in the membrane.

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  • 膜

    膜 32 (4), 221-228, 2007

    日本膜学会

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