Plaque formation and replication of porcine parvovirus in embryonic swine kidney cell line, ESK cells.

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  • 豚胎仔腎株化細胞における豚パルボウイルスのプラック形成と増殖
  • 豚胎仔腎株化細胞における豚パルボウイルスのプラック形成と増殖〔英文〕
  • ブタ タイシ ジン カブカ サイボウ ニ オケル ブタ パルボ ウイルス ノ

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Porcine parvovirus formed clear plaques on 3- and 24-hr-culture monolayer of embryonic swlie kidney cell line, ESK cells, by seeding with 3 ml of cell suspension containing 4×105 cells per ml in each well of the Costar's plate. Infective titer by plaque assay on 3-hr-culture monolayer was higher than that on 24-hr-culture monolayer. Plaques on 48- and 72-hr-culture monolayer were dull and uncountable. Plaques became large but infective titer did not increase when diethylaminoethyl-dextran was incorporated in the overlay medium. Plaque formation was specifically inhibited by antiserum against the virus. The virus grew well in 3-hr-culture monolayer and eclipse phase of the virus growth was between 8 and 10 hr.

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