【Original Contribution】 Reduction of Buffer Volume Used in Regeneration ofAnion–Exchange Porous Hollow–Fiber Membrane by Site–ControlledIntroduction of Anion–Exchange Group into Graft Chain
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- Nide Aguru
- Department of Applied Chemistry and Biotechnology
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- Kawai-Noma Shigeko
- Department of Applied Chemistry and Biotechnology
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- Umeno Daisuke
- Department of Applied Chemistry and Biotechnology
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- Saito Kyoich
- Department of Applied Chemistry and Biotechnology
Bibliographic Information
- Other Title
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- 【原著】 グラフト鎖へのアニオン交換基の導入部位の制御によるアニオン交換多孔性中空糸膜の再生に要する緩衝液量の削減
- グラフト鎖へのアニオン交換基の導入部位の制御によるアニオン交換多孔性中空糸膜の再生に要する緩衝液量の削減
- グラフト サ エ ノ アニオン コウカンキ ノ ドウニュウ ブイ ノ セイギョ ニ ヨル アニオン コウカン タコウセイ チュウクウ シマク ノ サイセイ ニ ヨウスル カンショウエキリョウ ノ サクゲン
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Abstract
A porous hollow–fiber membrane containing an anion–exchange graft chain is used as an adsorber for the removalof undesirable proteins. The adsorption and elution of the proteins are followed by the regeneration of the mem-brane. The volume of a buffer solution used for the regeneration of the anion–exchange porous hollow–fiber mem-brane was reduced by controlling the anion–exchange group distribution along the graft chain. First, glycidylmethacrylate(GMA)was graft-polymerized onto an electron–beam–irradiated porous hollow–fiber membrane.Second, diethylamino group was introduced into the epoxy group of the graft chain with water as a poor solvent forthe poly GMA chain. In a permeation mode, the membrane was regenerated with 1.0 M sodium hydroxide beforebeing rinsed with a phosphate buffer (pH 7.0). When the anion–exchange group was introduced preferentially intothe polymer brush, the buffer solution usage required to rinse the membrane to pH 7.2 was reduced by 40% com-pared to when the anion–exchange group was introduced uniformly along the graft chain. The binding capacity ofBSA to the polymer brush remained constant irrespective of the anion-exchange group distribution along the graft chain.
Journal
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- MEMBRANE
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MEMBRANE 39 (4), 258-263, 2014
THE MEMBRANE SOCIETY OF JAPAN
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Keywords
Details 詳細情報について
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- CRID
- 1390001206423798144
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- NII Article ID
- 130005069435
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- NII Book ID
- AN0023215X
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- ISSN
- 18846440
- 03851036
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- NDL BIB ID
- 025736311
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed