牛白血病細胞の酵素組織化学的研究

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  • Enzyme histochemical studies on bovine leukemia cells.
  • 牛白血病細胞の酵素組織化学的研究〔英文〕
  • ウシ ハッケツビョウ サイボウ ノ コウソ ソシキ カガクテキ ケンキュウ エ

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Enzyme histochemical activities of adenosin triphosphatase (ATPase) and acid phosphatase (AcPase) were studied on the superficial cervical lymph nodes in 18 cattle of bovine leukosis ( 12 adult form, 3 calf form, 3 thymic form). Similar tissues from 5 healthy cattle served as controls. ATPase gave a brown positive reaction corresponding to the lymphocyte membrane in the B lymphocyte dependent area. In cytoplasms of lymphocyte in the paracortex of T lymphocyte dependent area, accumulation of dark-brown granules indicating AcPase activity was noted. In 10 leukemic cattle of adult form, the neoplastic cells definitely revealed ATPase activity. Neoplastic cells of 6 cattle of thymic and calf form rarely revealed ATPase activity, and all exhibited specific reactions by the accumulation of AcPase positive granules. Accumulation of AcPase positive granules was demonstrated as clustered dense body electron microscopically.

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