ミラルディア(Millardia meltada)における寄生虫特異抗体測定のためのELISA法
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- TATEISHI Mika
- Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University
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- HORII Yoichiro
- Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University
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- MARUYAMA Haruhiko
- Department of Parasitology, Miyazaki Medical College
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- NAWA Yukifumi
- Department of Parasitology, Miyazaki Medical College
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- TSUCHIYA Kimiyuki
- Department of Experimental Animal Center, Miyazaki Medical College
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- MAKIMURA Susumu
- Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University
書誌事項
- タイトル別名
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- Sensitive Enzyme-Linked Immunosorbent Assay(ELISA) to Measure Parasite-Specific Antibodies of Indian Soft-Furred Rats, Millardia meltada.
- Sensitive enzyme-linked immunosorbent a
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抄録
Immunoglobulin G (IgG) of Indian soft-furred rat, Millardia meltada, was purified by an immunoaffinity chromatography and antibodies against it was raised in rabbit. Using this rabbit anti-M. meltada IgG antibody, sensitivity of enzyme-linked immunosorbent assay (ELISA) to measure parasite-specific antibodies in the sera of M. meltada was markedly enhanced than the previous method using rabbit anti-mouse IgG and rabbit anti-rat IgG antibodies, which could cross-react to M. meltada IgG. Since M. meltada could effectively produce circulating antibodies against two intestinal helminths, Strongyloides venezuelensis and Nippostrongylus brasiliensis, the high susceptibility of this animal to an array of parasites seems to be not due to general immunological deficiency.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 59 (6), 491-494, 1997
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206424310784
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- NII論文ID
- 110003915189
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK2sXkslahtLo%3D
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- ISSN
- 13477439
- 09167250
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- HANDLE
- 10458/1924
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- NDL書誌ID
- 4249579
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- PubMed
- 9234230
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可