Construction of the Recombinant Feline Herpesvirus Type 1 Deleted Thymidine Kinase Gene.

DOI Web Site Web Site PubMed 3 Citations 45 References

Bibliographic Information

Other Title
  • チミジンキナーゼ遺伝子を欠損させた組換え猫ヘルペスウイルス1型の作出
  • チミジンキナーゼ遺伝子を欠損させた組換え猫ヘルペスウイルス1型の作出〔英文〕
  • チミジン キナーゼ イデンシ オ ケッソンサセタ クミカエ ネコ ヘルペスウ

Search this article

Abstract

We constructed a recombinant virus containing defined deletion within thymidine kinase (TK) gene from feline herpesvirus type 1 (FHV-1) C7301, which was isolated in Japan, by standard rescue procedures with thymidine arabinoside (araT) selection. The araT resistant recombinant virus was designated as C7301dlTK. Southern blot analysis, together with polymerase chain reaction, revealed a deletion with the expected size, based on agarose gel electrophoresis, of the TK gene region in the C7301dlTK. Growth kinetics of the C7301dlTK in CRFK cells was similar to that of the parent C7301 that possesses TK activity. However, plaques produced by the C7301dlTK were significantly smaller than those of the parent virus. This report might be applicable for the development of a new genetically engineered FHV- 1 vaccine.

Journal

Citations (3)*help

See more

References(45)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top