Transcriptional Analysis of Marek's Disease Virus(MDV) Genes in MDV-Transformed Lymphoblastoid Cell Lines without MDV-Activated Cells.
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- UI Masahiro
- Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University
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- ENDOH Daiji
- Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University
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- CHO Kyoung-Oh
- Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University
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- KON Yasuhiro
- Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido University
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- IWATA Akira
- Nippon Institute of Biological Science
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- MAKI Yoshiyuki
- Nippon Genosis
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- SATO Fumiaki
- Institute for Environmental Science
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- KUWABARA Mikinori
- Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University
Bibliographic Information
- Other Title
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- Transcriptional Analysis of Marek′s Disease Virus(MDV)Genes in MDV-Transformed Lymphoblastoid Cell Lines without MDV-Activated Cells
- Transcriptional Analysis of Marek s Dis
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Abstract
Spontaneously activated MDV is rarely included in MDV-transformed cells, while it may influence the result of transcriptional analysis. A population consisting of 103 MDV-transformed cells probably did not include spontaneously activated MDV, since the estimated frequency of MDV-transformed cells including activated MDV was below 0.01% according to limiting-dilution polymerase chain reaction (PCR) and the presence of the major early antigen pp38 in 6 transformed cell lines. Reverse transcriptase-PCR (RT-PCR) products corresponding to ICP27, pol, TK, US3, A41, gA, gB and UL50 genes were undetectable in 103 cells by Southern hybridization of the RT-PCR products. Transcripts of the VP16 and SORF2 genes were detected in the 103 cells of MSB-1, and the pp14 gene transcript was found in 103 cells of RPL-1 but not in 103 cells of HPRS-1, MOGA-1, MOGA-2, MSB-1 or MTB-1. A transcript corresponding to the ICP4 sequence was detected as a 0.7 kbp RT-PCR product in 103 cells of these MDV cell lines but not in the retrovirus-transformed 1104B1 cell line. The transcript corresponding to the 0.7 kbp RT-PCR product suggested a splice by its size and sequence. Thus, transcriptional analysis of 103 MDV-transformed cells revealed that the transcript corresponding to the ICP4 sequence was a common transcript in latently infected MDV-transformed cells, while most of the genes did not transcribe in these cells.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 60 (7), 823-829, 1998
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206424593024
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- NII Article ID
- 110003918504
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DyaK1cXlsFCntLo%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 4523727
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- PubMed
- 9713810
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed