Molecular Cloning of Equine Chromogranin A and Its Expression in Endocrine and Exocrine Tissues.
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- SATO Fumio
- Laboratory of Molecular and Cellular Biology,Equine Research Institute,Japan Racing Association,321-4 Tokami-cho,Utsunomiya 320-0856,Japan
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- HASEGAWA Telhisa
- Laboratory of Molecular and Cellular Biology,Equine Research Institute,Japan Racing Association,321-4 Tokami-cho,Utsunomiya 320-0856,Japan
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- KATAYAMA Yoshinari
- Laboratory of Molecular and Cellular Biology,Equine Research Institute,Japan Racing Association,321-4 Tokami-cho,Utsunomiya 320-0856,Japan
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- IWANAGA Toshihiko
- Laboratory of Anatomy,Graduate School of Veterinary Medicine,Hokkaido University,Kita-18 Nishi-9,Kita-ku,Sapporo 060-0818,Japan
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- YANAIHARA Noboru
- Research and Development,Yanaihara Institute,Inc.,2480-1 Awakura,Fujinomiya 418-0011,Japan
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- KANNO Tomio
- Research and Development,Yanaihara Institute,Inc.,2480-1 Awakura,Fujinomiya 418-0011,Japan
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- ISHIDA Nobushige
- Laboratory of Molecular and Cellular Biology,Equine Research Institute,Japan Racing Association,321-4 Tokami-cho,Utsunomiya 320-0856,Japan
Bibliographic Information
- Other Title
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- 分子生物学:馬クロモグラニンA遺伝子のクローニングおよび内分泌腺と外分泌腺における発現
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Abstract
Chromogranin A(CGA)is a member of a family of highly acidic proteins co−stored and co−released with catecholamines in the adrenal medullary cells as well as in other neurons and paraneurons.The nucleotide sequence encoding equine CGA was determined using RT−PCR and rapid amplification of complementary DNA(cDNA)ends(RACE)techniques.A total 1, 828 bp of the nucleotide sequence reveals that equine CGA is a 448−residue protein preceded by an 18−residue signal peptide.Comparison of the amino acid sequence of equine CGA with those of human, porcine, bovine, mouse, rat and frog CGA showed high conservation at the NH2−terminal 1−77 amino acids regions(94.8%, 93.5%, 92.2%, 81.8%, 83.1% and 66.2%, respectively)and COOH−terminal 314−430 amino acids regions(90.6%, 81.4%, 90.6%, 80.5%, 83.3% and 39.0%, respectively), as well as a potential dibasic cleavage site, whereas the middle portion showed marked sequence variation(52.5%, 49.1%, 38.9%, 26.6%, 27.9% and 6.2%, respectively).Northern blot analysis and RT−PCR elucidated the tissue distribution of equine CGA mRNA.Its expression was confirmed not only in the adrenal medullary cells but also in other organs(cerebrum, cerebellum, pituitary gland, spinal cord, liver, thyroid gland, striated muscle, lung, spleen, kidney, parotid gland and sublingual gland).Further, in adrenal chromaffin cells and pituitary cells of the anterior−intermediate lobe, the expression was confirmed by in situ hybridization with anti−sense CGA cRNA probe.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 62 (9), 953-959, 2000
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206425075328
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- NII Article ID
- 110003920466
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3cXns1Grtrk%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 5528263
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- PubMed
- 11039590
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed