ACETYL CHOLINE RELEASE FROM THE PARTICULATE FRACTION OF RABBIT BRAIN

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  • 家兎脳顆粒分画よりの acetyl choline 遊離
  • カト ノウ カリュウ ブンカク ヨリ ノ acetyl choline ユウリ

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It has been well known that most of the acetyl choline (ACh) in the brain tissue is ininactive bound form, while a considerable amount of ACh is actually found in free form.It seems that some free ACh is released artificially from bound ACh during estimation.Recently it has been reported that most part of the bound ACh in the brain was distributedin the particulate fraction. It still remains obscure, however, whether ACh is incorporatedsimply in particles or bound chemically with them. It seems to be an effective aproachto these problems to develop an inproved estimating method with less artificial effect tostudy ACh release from particles in vitro. This paper deals with a method for estimationof free and bound ACh. It also describes the effects of some inorganic ions on the rateof ACh release from the brain particulate fraction in vitro. The results obtained aresummarized as follows. The rate of ACh release was lower in a O.32N1 sucrose solutioncontaining 5xlO M eserine salicyte at OC than in any other solution tested, i.e., acidalcohol or frog Ringers solution. The free ACh in the rabbit whole brain was assayeddirectly with the homogenate of the brain, using the rectus abdominis muscle. As a resttlt, it was about 30% of total ACh. A hypertonic sucrose solution had only a small effect onthe rate of ACh release, as well as an isotonic sucrose solution, while a hypotonic sucrosesolution had an increasing effect on it. A Nail solution showed the same effect oftonicity on the rate of ACh release as the sucrose solution. Within a range of 0.32 to 0.62in tonicity, potassium ion in a high concentration had an increasing effect on the rate of AChrelease, while calciurn and magnesium tons ltad a small or no and sodium Ton no effect on it. Allthese tons, even potassium ion, had no effect in Ringers type concentratiorn. From theseresults, it might be assumed that ACh was not simply incorporated in particles but was bound to them, and that it was released from them by dilution and the presence ofpotassium ion.

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