豚および牛から分離された Actinomyces pyogenes によって産生されるプロテアーゼのザイモグラムによる解析
書誌事項
- タイトル別名
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- Assay of Proteases from Actinomyces pyogenes Isolated from Pigs and Cows by Zymography.
- 豚および牛から分離されたActinomyces pyogenesによって産生されるプロテアーゼのザイモグラムによる解析(短報)〔英文〕
- ブタ オヨビ ウシ カラ ブンリサレタ Actinomyces pyogene
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説明
Extracellular proteases of Actinomyces pyogenes were assayed by zymography on SDS-polyacrylamide gel with the concentrated culture supernatant of 7 isolates from pigs and cows. When gelatin was used as a substrate, three proteases with molecular weights of 69, 59 and 55 kilodalton (kDa) were detected in all the isolates from both the pigs and cows. In addition to these proteases, 108 and 102 kDa proteases were detected in the swine and bovine isolates respectively. No protease bands appeared, however, when the calcium ion was absent from the buffer solution of the zymography. When casein was used as a substrate, the bands of 69, 59 and 55 kDa proteases were present, but 108 and 102 kDa proteases were not detected in any of the isolates. The activity of all proteases was completely inhibited by phenylmethyl-sulfonyl fluoride and diisopropyl flurophosphate but not by other protease inhibitors. From this result, it seems likely that the five proteases produced by A. pyogenes, originating from pigs and cows, are serine proteases.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 57 (5), 977-979, 1995
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206425484800
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- NII論文ID
- 130003572204
- 110003916411
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 3644042
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- PubMed
- 8593319
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可