Detection of Rabies Virus RNA Isolated from Several Species of Animals in Brazil by RT-PCR.
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- ITO Mikako
- Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine
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- ITOU Takuya
- Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine
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- SAKAI Takeo
- Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine
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- SANTOS Marli F.C.
- Centro de Diagnósticos e Pesquisas Veterinárias, Agência Goiana de Desenvolvimento Rural e Fundiário, Secretaria de Agricultura e Pecuária do Estado de Goiás, Av. Anhanguera, 1077, Setor Leste Universitário, Goiânia
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- ARAI Yohko T.
- Department of Virology 1, National Institute of Infectious Disease
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- TAKASAKI Tomohiko
- Department of Virology 1, National Institute of Infectious Disease
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- KURANE Ichiro
- Department of Virology 1, National Institute of Infectious Disease
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- ITO Fumio H.
- Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária
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Abstract
Brain samples from different animal species including humans: five vampire bats, 14 cattle, 12 dogs, 11 cats, two horses, one pig, one sheep and three humans collected from various geographical regions of Brazil were found to be positive for rabies by means of the fluorescent antibody test (FAT) and the mouse inoculation test (MIT). The brain samples were retested for rabies by means of the reverse transcription and polymerase chain reaction (RT-PCR) with 2 primer sets (P1/P2 and RHN1/RHNS3), which amplified full or partial regions on the nucleoprotein (N) gene of the rabies virus, respectively. Brain samples from five vampire bats, 13 cattle, one horse and one sheep failed to yield PCR products when the RHN1/RHNS3 primer pair was used, but all brain samples successfully yielded the products when the P1/P2 primer pair was used. These results suggest that Brazilian rabies virus isolates could be principally divided into two populations according to genetic difference.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 63 (12), 1309-1313, 2001
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206425717376
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- NII Article ID
- 110003920795
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD38XlvFGitA%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 6016912
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- PubMed
- 11789609
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed