Norsalsolinol Uptake into Secretory Vesicles Via Vesicular Monoamine Transporter and Its Secretion by Membrane Depolarization or Purinoceptor Stimulation in PC12 Cells.

DOI Web Site Web Site PubMed 参考文献46件
  • MARUYAMA Yutaka
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
  • SUZUKI Yuko
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
  • KAZUSAKA Akio
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
  • FUJITA Shoichi
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University

この論文をさがす

抄録

The intracellular dynamics of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, in PC12 cells was studied. We found that dopamine and norsalsolinol are co-localized to secretory granule layer by sucrose density gradient in norsalsolinol-treated PC12 cells. The norsalsolinol was actively taken up into isolated secretory vesicle fraction from PC12 cells with a Km value of 41.5 ± 6.8 μM. The uptake of 10 μM of norsalsolinol was sensitive to reserpine (1 μM), an inhibitor of vesicular dopamine transporter, and dopamine, an endogenous substrate, but insensitive to GBR-12909, an inhibitor of dopamine transporter on plasma membrane. In norsalsolinol-treated PC12 cells, exposure to high K+ or ATP resulted in simultaneous release of norsalsolinol and dopamine. Time course of a release of dopamine and that of norsalsolinol evoked by 50 mM KCl or 100 μM ATP corresponded to each other. These releases were dependent on the concentrations of secretagogues. These data suggest that norsalsolinol is taken up with dopamine into secretory vesicle via vesicular catecholamine transporter.

収録刊行物

参考文献 (46)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ