Breed Differences in Genotype and Allele Frequency of Catechol O-Methyltransferase Gene polymorphic Regions in Dogs
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- MASUDA Koji
- Laboratory of Veterinary Ethology, The University of Tokyo
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- HASHIZUME Chie
- Laboratory of Veterinary Ethology, The University of Tokyo
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- KIKUSUI Takefumi
- Laboratory of Veterinary Ethology, The University of Tokyo
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- TAKEUCHI Yukari
- Laboratory of Veterinary Ethology, The University of Tokyo
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- MORI Yuji
- Laboratory of Veterinary Ethology, The University of Tokyo
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Catechol O-methyltransferase (COMT) inactivates catecholamines and catechol-containing drugs such as L-DOPA. The common genetic polymorphism Val158Met in the human COMT gene is suspected to be associated with "persistence" or risk for schizophrenia. In this study, we attempted to identify the canine COMT gene fragment and to find a similar polymorphism and to reveal its genetic distribution among five representative canine breeds. We found that the amplified gene consisted of 663 bp nucleotides and was 84% homologous with the human COMT gene. The single nucleotide polymorphisms, guanine adenine substitution, were observed at the 39th, 216th and 482nd nucleotides. From the genotyping of the 216th polymorphism among 266 dogs by the polymerase chain reaction-restriction fragment length polymorphism method with restriction enzyme EagI, and that of the 482nd polymorphism with restriction enzyme SfcI, we found inter-breed variations of genotypes as well as of allelic frequencies for both of these polymorphic regions. These results suggest that the identified polymorphisms will be useful tools in elucidating the genetic background of canine behavioral traits.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (2), 183-187, 2004
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426685312
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- NII論文ID
- 110000010609
- 110003886376
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6863622
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- PubMed
- 15031547
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可