Detection of Interleukin-1 and Interleukin-6 on Cryopreserved Bovine Mammary Epithelial Cells in Vitro.
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- OKADA Hiroyuki
- Department of Veterinary Pathology, Rakuno Gakuen University
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- ITO Takashi
- Hokkaido Branch, National Institute of Animal Health
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- OHTSUKA Hiromichi
- Department of Veterinary Internal Medicine, Rakuno Gakuen University
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- KIRISAWA Rikio
- Department of Veterinary Microbiology, Rakuno Gakuen University
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- IWAI Hiroshi
- Department of Veterinary Microbiology, Rakuno Gakuen University
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- YAMASHITA Kazuto
- Department of Veterinary Surgery, Rakuno Gakuen University
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- YOSHINO Tomo-o
- Department of Veterinary Pathology, Rakuno Gakuen University
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- ROSOL Thomas J.
- Department of Veterinary Biosciences, The Ohio State University
書誌事項
- タイトル別名
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- Detection of interleukin-1 and interleu
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This investigation was performed to determine whether primary cultures of mammary cells from lactating cows would sustain production of interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF), and express mRNA for cytokines interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, interferon (INF)-τ, TNF-α, and granulocyte-macrophage colony-stimulating factor (GM-CSF) by the reverse transcription-polymerase chain reaction (RT-PCR) in vitro. Cryopreserved mammary epithelial cells collected from cows at 1 week post calving were plated in collagen-coated 24-well culture plates (250,000 cells/well). IL-1 and IL-6 productions were measured using a A375 cell growth inhibition assay and a 7TD1 hybridoma proliferation assay, respectively. Production of IL-1 was demonstrated in mammary epithelial cells cultured with unsupplemented medium, but was not produced by cells cultured in medium supplemented with fetal bovine serum. IL-6 production in the conditioned medium was continued at steady level until day 14, whereas IL-6-like bioactivity was not detected in medium alone. TNF-like activity was not detectable in any experiments. This study also demonstrated the expression of mRNA for multiple cytokines including IL-1α, IL-1β, IL-6, IL-10, TNF-α, and GM-CSF by RT-PCR in mammary cell cultures. The results indicate that bovine mammary epithelial cells of lactating cows produce IL-1 and IL-6 and have gene expression for multiple cytokines. This in vitro model will be useful to investigate the function and regulation of IL-1 and IL-6 in the lactating mammary gland.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 59 (7), 503-507, 1997
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426697344
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- NII論文ID
- 110003915192
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK2sXlsFWqsrw%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 4258522
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- PubMed
- 9271442
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可