Production of HanWoo(Bos taurus coreanae) Fetuses Following Interbreed Somatic Cell Nuclear Transfer.
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- ROH Sangho
- Department of Animal Life and Resources, Hankyong National University
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- YOON Jong-taek
- Department of Animal Life and Resources, Hankyong National University
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The possibility of producing HanWoo (Bos taurus coreanae) calves from transferable bovine embryos, obtained by interbreed nuclear transfer using Holstein cytoplasts and surrogates, was investigated. As donor nuclei, HanWoo fetal fibroblasts were used. Cells were induced into quiescence by serum deprivation for 4-7 days before nuclear transfer. In vitro matured Holstein oocytes were enucleated, and single donor cell was placed into the perivitelline space of enucleated oocyte. After reconstruction, the embryos were fused, activated and cultured. On day 7, the embryos that developed to the blastocyst stages were transferred into Holstein recipient cows on day 6 to 7 of estrous cycle (estrus=0). The reconstructed embryos were successfully fused (58.8%; 47/80), cleaved (91.5%; 43/47), and developed to blastocysts (29.8%; 14/47). Eleven blastocysts were transferred into 5 Holstein recipient cows. Two recipients were pregnant, confirmed by ultrasonography at day 60 of gestation. But, one of them was opened between on day 80 to 100 of pregnancy, and the other had a stillbirth on day 255. The stillborn calf was physically normal, and we couldn't find any evidence of anomaly. These results show that cells derived from HanWoo somatic cell lines can be reprogrammed by interbreed nuclear transfer and develop subsequently in vivo as well as in vitro.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 63 (9), 945-948, 2001
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426769152
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- NII論文ID
- 110003920725
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BD3Mrmt1anug%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 5937886
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- PubMed
- 11642280
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可