鶏血漿中F-GOTの酵素作用の性質について (3) : F-GOT反応における速度と温度との関係

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  • Thermodynamics of Enzymatic Transamination of F-GOT in Normal Fowl Blood Plasma : III. Relationship between Temperature and Velocity in F-GOT Reaction
  • 鶏血漿中F-GOTの酵素作用の性質について : III. F-GOT反応における速度と温度との関係
  • ニワトリ ケッショウチュウ F GOT ノ コウソ サヨウ ノ セイシツ ニ

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The effect of temperature on F-GOT reaction was analyzed kinetically by varying temperature within a range from 0 to 60°C. The results obtained are summarized as follows. 1. The rate of F-GOT-catalyzed reaction increased gradually with a rise in temperature within a range from 4 to 40°C. It passed through a maximum at 50°C and decreased rapidly, if not precipitously, at a temperature between 56 and 60°C. On the contrary, the addition of pyidoxal 5'-phosphate (PALP) to such heat-treated preparations increased or restored the activity in part. The molar concentration of PALP required for maximal activity was 1×10-3M. An apparently maximal activation with PALP was also observed in preparations stabilized at a constant temperature of 20°C. 2. The GOT reaction in both directions met the condition of conventional first-order reactions. No special equilibrium, however, was recognized in an approach to equilibrium in the reaction in both directions. 8. The temperature coefficient Q10 varied from 1 to 4 at a temperature between 0 and 50°C. It increased by approximately 1.3 times for a rise of every 10°C. 4. Reactions took place a little later than usual when carried out at a temperature below 20°C. The reaction velocity, however, was found to increase continuously with a rise in temperature within a range from 4 to 40°C. The Arrhenius equation was used to express the relationship between the temperature concerned and the average value of energy of activation. When calculated from the slope of the straight line in Arrhenius' graph, this energy was 9, 024.18 calories per mole. On the other hand, when calculated from the log Vmax-vs.-1/T point at 50°C, the energy of activation showed a tendency to proceed toward inferiority, deviated from presenting any linear correlation. From these results, it was concluded that F-GOT might be stable and retain full activity at 40°C.

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