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- TAKABATAKE Noriyuki
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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- OKAMURA Masashi
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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- YOKOYAMA Naoaki
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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- OKUBO Kazuhiro
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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- IKEHARA Yuzuru
- Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology
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- IGARASHI Ikuo
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
書誌事項
- タイトル別名
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- Involvement of a Host Erythrocyte Sialic Acid Content in Babesia bovis Infection
- Parasitology: Involvement of a host erythrocyte sialic acid content in Babesia bovis infection
この論文をさがす
抄録
Host sialic acid (SA) has recently been suggested to play an important role in erythrocyte (RBC) infection by Babesia spp. The present study attempted to further determine the specific type of SAs important in the RBC invasion. Bovine RBC was found to bear abundant α2-3-linked SA residues but not α2-6-linked SA in nature, confirmed by flow cytometric analysis of the neuraminidase (Nm)-treated RBCs. Lectin-blot analyses revealed the removal of α2-3-linked SAs from the 97-, 33-, and 31-kDa bands by the Nm treatment. Addition of the Nm-treated RBCs into an in vitro culture of B. bovis resulted in a decreased population of the parasitized RBCs. The thin smear samples from the cultures were then observed under a confocal laser scanning microscope after staining with the α2-3-linked SA-specific lectin: a selective invasion of B. bovis was found only in the intact RBCs bearing the SAs, but not in the desialylated RBCs. Furthermore, a significant reduction of the parasitized RBCs was also observed in the culture supplemented with exogenous 3'-sialyllactose containing the α2-3-linked SAs. However, the complete inhibition of parasite proliferation was not achieved in the culture. These findings indicate that while the α2-3-linked SA-dependent pathway is needed for highly efficient invasion of host RBCs by B. bovis, there might also be other potential alternative pathways.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 69 (10), 999-1004, 2007
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206427716352
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- NII論文ID
- 110006418717
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 8985444
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可