Bovine viral diarrhea virus 2 Infection Activates the Unfolded Protein Response in MDBK Cells, Leading to Apoptosis
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- MAEDA Kouji
- Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University
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- FUJIHARA Masatoshi
- Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University
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- HARASAWA Ryô
- Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University
Bibliographic Information
- Other Title
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- Virology: Bovine viral diarrhea virus 2 infection activates the unfolded protein response in MDBK cells, leading to apoptosis
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Abstract
Bovine viral diarrhea virus 2 (BVDV-2) strains are divided into cytopathic and non-cytopathic biotypes based on the ablity to induce cytopathic effects in cultured cells. The mechanism of cytopathogenicity of BVDV-2 is not well understood. We examined cytopathogenesis in MDBK cells resulting from BVDV-2 infections by microscopic examinations and microarray analysis. We found that BVDV-2 activates endoplasmic reticulum (ER) stress signaling pathways that contribute to apoptosis of infected cells. We also monitored the expression of ER stress marker gene by RT-PCR during BVDV-2 infection and demonstrated that infection of MDBK cells with a cytopathic strain of BVDV-2 induces glucose-regulated protein 78 expression. Infection with BVDV-2 also induces DNA-damage-inducible transcript 3 expression and downregulates the lectin-galactoside-binding soluble 1 level. These results show that cytopathic strains of BVDV-2 induce an ER stress response resulting in apoptosis.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 71 (6), 801-805, 2009
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206428432768
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- NII Article ID
- 130000134407
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 10349853
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed