Further evaluation of an ELISA kit for detection of antibodies to a nonstructural protein of foot-and-mouth disease virus
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- FUKAI Katsuhiko
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- NISHI Tatsuya
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- MORIOKA Kazuki
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- YAMADA Manabu
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- YOSHIDA Kazuo
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- KITANO Rie
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- YAMAZOE Reiko
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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- KANNO Toru
- Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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説明
An ELISA kit for detection of antibodies to a nonstructural protein of foot-and-mouth disease (FMDV) was further evaluated using sequentially collected serum samples of experimentally infected animals, because the sensitivity of the kit used in a previous study was significantly low in field animals. The kit fully detected antibodies in infected animals without vaccination; however, the first detections of antibodies by the kit were later than those by the liquid-phase blocking ELISA that is used for serological surveillance in the aftermath of outbreaks in Japan, for detection of antibodies to structural proteins of FMDV. Additionally, although the kit effectively detected antibodies in infected cattle with vaccination, there were several infected pigs with vaccination for which the kit did not detect antibodies during the experimental period. Taken together, the kit may not be suitable for serological surveillance after an FMD outbreak either with or without emergency vaccination in FMD-free countries.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 78 (3), 365-373, 2016
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206428588544
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- NII論文ID
- 130005140909
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 027226682
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- PubMed
- 26498533
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可