Hormonal Differences in Peripheral Blood and Gene Profiling in the Liver and Lymphocytes in Japanese Black Cattle with Growth Retardation

DOI Web Site Web Site PubMed 被引用文献1件
  • ISHIDA Sakura
    Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • YONEZAWA Tomohiro
    Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • EIRAI Sayoko
    Nara Prefectural Livestock Hygiene Service Center, 152–1 Minamijyuso, Gose, Nara 639–2204, Japan
  • KIZAKI Keiichiro
    Laboratory of Veterinary Physiology, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
  • HASHIZUME Kazuyoshi
    Laboratory of Veterinary Physiology, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
  • TOMIOKA Michiko
    Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • KUROSE Yohei
    Laboratory of Animal Nutrition, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • HIRANO Takashi
    Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Fukushima 961–8061, Japan
  • WATANABE Daisaku
    Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

この論文をさがす

抄録

Japanese Black cattle occasionally demonstrate growth retardation despite sufficient nutrient intake. To clarify hormonal and transcriptional characteristics, we investigated differences in blood components, including hormones, and differences in exhaustive gene expressions in the liver and peripheral lymphocytes of six cattle with growth retardation (GR cattle) and eight control cattle of the same age and pedigree with normal growth. Hematocrit values and concentrations of hemoglobin, serum albumin, total cholesterol, insulin-like growth factor 1 (IGF-1), thyroxine and insulin in GR cattle were significantly lower than those in controls. GR cattle also excreted higher levels of GH. We used three GR and three control cattle for a microarray analysis in the liver and found that 279 gene expressions were significantly different. However, gene expressions related to the GH-IGF-1 axis, such as the GH receptor and IGF-1, were not significantly different from those of controls. Immune-related gene expressions were significantly lower. To clarify these gene expression levels, peripheral lymphocytes were used for real-time RT-PCR. The expression rates of genes that were significantly lower in the liver, such as chemokine ligand 8, interferon gamma receptor 1 and immunoglobulin light chain VJ region were also significantly lower in three GR cattle than those in the three control cattle. These results suggest that the cause of growth retardation in the present study was due to other factors, not abnormal gene expressions of factors related to the GH-IGF-1 axis in the liver, and that GR cattle were susceptible to infectious disease.

収録刊行物

被引用文献 (1)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ