エクアドルのリーシュマニア症流行地におけるサシチョウバエの遺伝子タイピング法の確立

DOI 機関リポジトリ 日本農学文献記事索引 Web Site Web Site 被引用文献7件 参考文献40件
  • TERAYAMA Yoshimi
    Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University
  • KATO Hirotomo
    Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University
  • GOMEZ Eduardo A.
    Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica
  • UEZATO Hiroshi
    Department of Dermatology, Faculty of Medicine, University of the Ryukyus
  • CALVOPIÑA Manuel
    Department of Parasitology, Kochi Medical School, Kochi University
  • IWATA Hiroyuki
    Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University
  • HASHIGUCHI Yoshihisa
    Department of Parasitology, Kochi Medical School, Kochi University

書誌事項

タイトル別名
  • Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene
  • Parasitology: Molecular typing of sand fly species (Diptera, Psychodidae, Phlebotominae) from areas endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S ribosomal RNA gene
  • Molecular typing of sand fly species from areas endemic for leishmaniasis in Ecuador by PCR-RFLP of 18S ribosomal RNA gene

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抄録

Surveillance of the distribution of sand fly species is important for prediction of the risk and expansion of Leishmania infection in endemic and surrounding areas. In the present study, a simple and reliable method of typing New World Lutzomyia species circulating in endemic areas in Ecuador was established by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique. PCR-RFLP of 18S ribosomal RNA (rRNA) genes with the restriction enzyme AfaI and subsequently HinfI successfully identified seven sand fly species in nine endemic areas in Ecuador. Although intraspecific genetic-diversity affecting the RFLP-patterns was detected in a species, the patterns were species specific. The method promises to be a powerful tool for the classification of New World Lutzomyia species.<br>

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