A Case Report Concerning Male Gametes Rescued from a Siamese Eld's Deer (Rucervus eldii siamensis) : Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability
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- THUWANUT Paweena
- Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
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- THONGPHAKDEE Ampika
- Zoological Park Organization under the Royal Patronage of H. M. the King, Bangkok 10300, Thailand
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- SOMMANUSTWEECHAI Angkana
- Zoological Park Organization under the Royal Patronage of H. M. the King, Bangkok 10300, Thailand
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- SIRIAROONRAT Boripat
- Zoological Park Organization under the Royal Patronage of H. M. the King, Bangkok 10300, Thailand
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- CHATDARONG Kaywalee
- Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
書誌事項
- タイトル別名
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- A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability
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抄録
In the present study, the quality of frozen-thawed epididymal and testicular sperm recovered from a Siamese Eld’s deer was examined. The epididymal sperm quality was assessed in fresh, cold-stored at 4°C and frozen-thawed samples. Zona binding ability of the frozen-thawed epididymal samples with Burmese Eld’s deer oocytes was also evaluated. Testicular sperm extracted from tissues frozen at -80 or -196°C for one month were examined for membrane and DNA integrity. Epididymal sperm retained their quality for up to 24 hr of cold storage at 4°C. The percentages of sperm motility, intact membrane, intact acrosome and intact DNA were 30, 46.5, 27 and 89.5% in the frozen and thawed epididymal sperm, and the average ability to bind with oocytes was 92.5 ± 64 sperm/oocytes. Around 70% of the sperm extracted from testicular tissues cryopreserved at -196 and -80°C for one month showed an intact membrane. In conclusion, epididymal and testicular sperm survived for more than 13 hr post-mortem. Furthermore, cold storage at 4°C and cryopreservation at -196 and -80°C maintain the quality of epididymal and testicular sperm. This study represents a model for male gamete rescue in endangered Eld’s deer.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 75 (1), 123-125, 2013
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206428750848
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- NII論文ID
- 130001879654
- 40019570078
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC38bmtlKjug%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 024246419
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- PubMed
- 22971799
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可