Internal Medicine : Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

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  • CHIKAZAWA Seishiro
    Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • HORI Yasutomo
    Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • HOSHI Fumio
    Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • KANAI Kazutaka
    Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • ITO Naoyuki
    Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • SATO Jun
    Tomizawa Animal Hospital, 2–1–30 Tomizawa Taihaku-ku, Sendai, Miyagi 982–0032, Japan
  • ORINO Koichi
    Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • WATANABE Kiyotaka
    Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • HIGUCHI Sei-ichi
    Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

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  • Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

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We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.

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