Bacteriology: Antimicrobial resistance and genetic characteristics of Salmonella Typhimurium isolated from horses in Hokkaido, Japan

DOI Web Site Web Site 被引用文献3件 参考文献22件
  • NIWA Hidekazu
    Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association
  • ANZAI Toru
    Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association
  • IZUMIYA Hidemasa
    Department of Bacteriology, National Institute of Infectious Diseases
  • MORITA-ISHIHARA Tomoko
    Department of Bacteriology, National Institute of Infectious Diseases
  • WATANABE Haruo
    Department of Bacteriology, National Institute of Infectious Diseases
  • UCHIDA Ikuo
    Hokkaido Research Station, National Institute of Animal Health
  • TOZAKI Teruaki
    Department of Molecular Genetics, Laboratory of Racing Chemistry
  • HOBO Seiji
    Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association

書誌事項

タイトル別名
  • Antimicrobial Resistance and Genetic Characteristics of Salmonella Typhimurium Isolated from Horses in Hokkaido, Japan

この論文をさがす

抄録

In this study, we examined the antimicrobial susceptibility of 16 Salmonella Typhimurium isolates obtained from horses, and applied several genetic methods, namely polymerase chain reaction (PCR) for detecting class 1 integrons, multiplex PCR for detecting multidrug resistant S. Typhimurium definitive phage type 104 (MR-DT104), and fluorescent amplified-fragment length polymorphism (FAFLP). Seven isolates with an ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline (ACSSuT) type resistance pattern, harbored two class 1 integrons with sizes of 1.2 and 1.0 kb, and were identified as DT104 by bacteriophage typing. These isolates also showed a typical MR-DT104 amplification pattern, which was positive for flost, spvC, invA and int, in multiplex PCR. In the FAFLP analysis, the equine DT104 isolates and the previously reported ACSSuT-type resistant bovine isolates, which were also isolated in Hokkaido were included in the same genetic cluster. Our results retrospectively indicate that MR-DT104 infection has existed in horses in Japan at least since 1996, and it was suggested that there is a highly epidemiological relationship between the equine MR-DT104 isolates and certain multidrug resistant bovine isolates in the same area. <br>

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (22)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ