Luteolin attenuates endotoxin-induced uveitis in Lewis rats
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- KANAI Kazutaka
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- HATTA Takuya
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- NAGATA Sho
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- SUGIURA Yuichi
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- SATO Kazuaki
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- YAMASHITA Yohei
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- KIMURA Yuya
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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- ITOH Naoyuki
- Department of Small Animal Internal Medicine I, School of Veterinary Medicine, University of Kitasato, Towada, Aomori 034–8628, Japan
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<p>The aim of the present study was to investigate the efficacy of luteolin on endotoxin-induced uveitis (EIU) in rats. EIU was induced in Lewis rats by subcutaneous injections of lipopolysaccharide (LPS). One hr before the LPS injection, 0.1, 1 or 10 mg/kg luteolin or 1 mg/kg prednisolone was intraperitoneally injected. We investigated its effect upon clinical scores, cellular infiltration and protein leakage, as well as on the level of tumor necrosis factor (TNF)-α, nitric oxide (NO) and prostaglandin (PG) E2 in the aqueous humor (AqH). Histologic examination and immunohistochemical analysis in the iris-ciliary body (ICB) were performed to determine the expressions of cyclooxygenase (COX)-2 and inducible NO synthase (iNOS), and then the activated nuclear factor (NF)-κB p65, I kappa B (IκB)-α degradation, phosphorylated (p)-IκB kinase (IKK) α/β and activator protein (AP)-1 c-Jun. Luteolin suppressed, in a dose-dependent manner, the clinical scores, number of inflammatory cells, the protein concentration, and the TNF-α, NO and PGE2 levels in the AqH and improved the histiologic status of the ocular tissue. Luteolin suppressed the expression of iNOS and COX-2 and the activated NF-κB p65, IκB-α degradation, p-IKKα/β and AP-1 p-c-Jun in the ICB. The anti-inflammatory potency of 10 mg/kg luteolin was as strong as that observed with 1 mg/kg prednisolone. These results demonstrate that luteolin attenuates ocular inflammation by inhibiting expression and release of inflammatory markers, along with the inhibition of the activated NF-κB pathway and at least partly AP-1 activity in the ICB.</p>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 78 (8), 1229-1235, 2016
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206429210368
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- NII論文ID
- 130005261518
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 027625114
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- PubMed
- 27098110
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可